uni-leipzig-open-access/json/s13567-023-01172-y

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The interaction of the parasite with the intestinal host cells has not been characterized thoroughly yet but may be affected by the nutritional demand of the parasite. Hence, we aimed to investigate the impact of <jats:italic>C. parvum<\/jats:italic> infection on glucose metabolism in neonatal calves. Therefore, <jats:italic>N<\/jats:italic>\u2009=\u20095 neonatal calves were infected with <jats:italic>C. parvum<\/jats:italic> on the first day of life, whereas a control group was not (<jats:italic>N<\/jats:italic>\u2009=\u20095). The calves were monitored clinically for one week, and glucose absorption, turnover and oxidation were assessed using stable isotope labelled glucose. The transepithelial transport of glucose was measured using the Ussing chamber technique. Glucose transporters were quantified on gene and protein expression level using RT-qPCR and Western blot in the jejunum epithelium and brush border membrane preparations. Plasma glucose concentration and oral glucose absorption were decreased despite an increased electrogenic phlorizin sensitive transepithelial transport of glucose in infected calves. No difference in the gene or protein abundance of glucose transporters, but an enrichment of glucose transporter 2 in the brush border was observed in the infected calves. Furthermore, the mRNA for enzymes of the glycolysis pathway was increased indicating enhanced glucose oxidation in the infected gut. In summary, <jats:italic>C. parvum<\/jats:italic> infection modulates intestinal epithelial glucose absorption and metabolism. We assume that the metabolic competition of the parasite for glucose causes the host cells to upregulate their uptake mechanisms and metabolic machinery to compensate for the energy losses.<\/jats:p>","DOI":"10.1186\/s13567-023-01172-y","type":"journal-article","created":{"date-parts":[[2023,5,3]],"date-time":"2023-05-03T21:06:52Z","timestamp":1683148012000},"update-policy":"http:\/\/dx.doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Cryptosporidium parvum competes with the intestinal epithelial cells for glucose and impairs systemic glucose supply in neonatal calves"],"prefix":"10.1186","volume":"54","author":[{"ORCID":"http:\/\/orcid.org\/0000-0002-2178-2270","authenticated-orcid":false,"given":"Franziska","family":"Dengler","sequence":"first","affiliation":[]},{"given":"Harald M.","family":"Hammon","sequence":"additional","affiliation":[]},{"given":"Wendy","family":"Liermann","sequence":"additional","affiliation":[]},{"given":"Solvig","family":"G\u00f6rs","sequence":"additional","affiliation":[]},{"given":"Lisa","family":"Bachmann","sequence":"additional","affiliation":[]},{"given":"Christiane","family":"Helm","sequence":"additional","affiliation":[]},{"given":"Reiner","family":"Ulrich","sequence":"additional","affiliation":[]},{"given":"Cora","family":"Delling","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2023,5,3]]},"reference":[{"key":"1172_CR1","doi-asserted-by":"publisher","first-page":"209","DOI":"10.1016\/S0140-6736(13)60844-2","volume":"382","author":"KL Kotloff","year":"2013","unstructured":"Kotloff KL, Nataro JP, Blackwelder WC, Nasrin D, Farag TH, Panchalingam S, Wu Y, Sow SO, Sur D, Breiman RF, Faruque ASG, Zaidi AKM, Saha D, Alonso PL, Tamboura B, Sanogo D, Onwuchekwa U, Manna B, Ramamurthy T, Kanungo S, Ochieng JB, Omore R, Oundo JO, Hossain A, Das SK, Ahmed S, Qureshi S, Quadri F, Adegbola RA, Antonio M, et al. 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