1 line
No EOL
14 KiB
Text
1 line
No EOL
14 KiB
Text
{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,8,21]],"date-time":"2023-08-21T04:53:58Z","timestamp":1692593638362},"reference-count":79,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,1,15]],"date-time":"2023-01-15T00:00:00Z","timestamp":1673740800000},"content-version":"vor","delay-in-days":14,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["RTG 2341"]},{"DOI":"10.13039\/501100004350","name":"Studienstiftung des Deutschen Volkes","doi-asserted-by":"publisher","award":["PhD fellowship"]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Plant Direct"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Old Yellow Enzymes (OYEs) are flavin\u2010containing ene\u2010reductases that have been intensely studied with regard to their biotechnological potential for sustainable chemical syntheses. OYE\u2010encoding genes are found throughout the domains of life, but their physiological role is mostly unknown, one reason for this being the promiscuity of most ene\u2010reductases studied to date. The unicellular green alga <jats:styled-content style=\"fixed-case\"><jats:italic>Chlamydomonas reinhardtii<\/jats:italic><\/jats:styled-content> possesses four genes coding for OYEs, three of which we have analyzed biochemically before. Ene\u2010reductase CrOYE3 stood out in that it showed an unusually narrow substrate scope and converted <jats:italic>N<\/jats:italic>\u2010methylmaleimide (NMI) with high rates. This was recapitulated in a <jats:styled-content style=\"fixed-case\"><jats:italic>C. reinhardtii<\/jats:italic><\/jats:styled-content> <jats:italic>croye3<\/jats:italic> mutant that, in contrast to the wild type, hardly degraded externally added NMI. Here we show that CrOYE3\u2010mediated NMI conversion depends on electrons generated photosynthetically by photosystem II (PSII) and that the <jats:italic>croye3<\/jats:italic> mutant exhibits slightly decreased photochemical quenching in high light. Non\u2010photochemical quenching is strongly impaired in this mutant, and it shows enhanced oxidative stress. The phenotypes of the mutant suggest that <jats:styled-content style=\"fixed-case\"><jats:italic>C. reinhardtii<\/jats:italic><\/jats:styled-content> CrOYE3 is involved in the protection against photooxidative stress, possibly by converting reactive carbonyl species derived from lipid peroxides or maleimides from tetrapyrrole degradation.<\/jats:p>","DOI":"10.1002\/pld3.480","type":"journal-article","created":{"date-parts":[[2023,1,16]],"date-time":"2023-01-16T00:22:05Z","timestamp":1673828525000},"update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["<scp><i>Chlamydomonas reinhardtii<\/i><\/scp> mutants deficient for Old Yellow Enzyme 3 exhibit increased photooxidative stress"],"prefix":"10.1002","volume":"7","author":[{"ORCID":"http:\/\/orcid.org\/0000-0002-1675-0936","authenticated-orcid":false,"given":"Stefanie","family":"B\u00f6hmer","sequence":"first","affiliation":[{"name":"Faculty of Biology and Biotechnology, Photobiotechnology Ruhr University Bochum Bochum Germany"}]},{"ORCID":"http:\/\/orcid.org\/0000-0002-0698-0674","authenticated-orcid":false,"given":"Christina","family":"Marx","sequence":"additional","affiliation":[{"name":"SolarBioproducts Ruhr Business Development Agency Herne Herne Germany"}]},{"ORCID":"http:\/\/orcid.org\/0000-0001-9347-8158","authenticated-orcid":false,"given":"Reimund","family":"Goss","sequence":"additional","affiliation":[{"name":"Institute of Biology, Plant Physiology Leipzig University Leipzig Germany"}]},{"given":"Matthias","family":"Gilbert","sequence":"additional","affiliation":[{"name":"Institute of Biology, Plant Physiology Leipzig University Leipzig Germany"}]},{"ORCID":"http:\/\/orcid.org\/0000-0002-2226-845X","authenticated-orcid":false,"given":"Severin","family":"Sasso","sequence":"additional","affiliation":[{"name":"Institute of Biology, Plant Physiology Leipzig University Leipzig Germany"}]},{"ORCID":"http:\/\/orcid.org\/0000-0003-1206-5234","authenticated-orcid":false,"given":"Thomas","family":"Happe","sequence":"additional","affiliation":[{"name":"Faculty of Biology and Biotechnology, Photobiotechnology Ruhr University Bochum Bochum Germany"}]},{"ORCID":"http:\/\/orcid.org\/0000-0001-8879-3348","authenticated-orcid":false,"given":"Anja","family":"Hemschemeier","sequence":"additional","affiliation":[{"name":"Faculty of Biology and Biotechnology, Photobiotechnology Ruhr University Bochum Bochum Germany"}]}],"member":"311","published-online":{"date-parts":[[2023,1,15]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.112.108274"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.24.1.1"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M115.704601"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M312919200"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1078\/14344610260450136"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2021.720867"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/catal7080240"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.algal.2020.101970"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.1000577"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20050979"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.18.00721"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.17.00421"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbabio.2015.02.008"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11120-013-9859-5"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/1873-3468.12833"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/tpj.12825"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1116843109"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M211778200"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004250100589"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phytochem.2019.03.002"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1078\/0176-1617-01369"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1809812116"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbi.2015.04.009"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jplph.2014.03.004"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.201500667"},{"key":"e_1_2_10_27_1","first-page":"378","article-title":"Isolation of a new yellow enzyme","volume":"298","author":"Haas E.","year":"1938","journal-title":"Biochemische Zeitschrift"},{"key":"e_1_2_10_28_1","volume-title":"The Chlamydomonas sourcebook: A comprehensive guide to biology and laboratory use","author":"Harris E. H.","year":"1989"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/1751-7915.13526"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/cctc.202002011"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.49.32763"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1093\/pcp\/pcz022"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201601200"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/chem.202103949"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1139\/cjb-2021-0032"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00425-018-3048-x"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41588-019-0370-6"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/nplants.2016.140"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/tpj.12787"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plaphy.2012.03.010"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/ert208"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gca.2013.06.004"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.25.14162"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1005936823310"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature08587"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1002\/slct.201700724"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.201800770"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.4161\/psb.26655"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1002\/chem.201905236"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1111\/ppl.12567"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/eraa022"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2018.03.030"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-019-10287-2"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/0926-6550(63)90386-4"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.18.00952"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02637260"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1997.t01-1-00294.x"},{"key":"e_1_2_10_58_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.44.28066"},{"key":"e_1_2_10_59_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.02410"},{"key":"e_1_2_10_60_1","doi-asserted-by":"publisher","DOI":"10.3390\/catal7050130"},{"key":"e_1_2_10_61_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-020-10845-z"},{"key":"e_1_2_10_62_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms5150"},{"key":"e_1_2_10_63_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.190264497"},{"key":"e_1_2_10_64_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2007.02.025"},{"key":"e_1_2_10_65_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.pcp.a029622"},{"key":"e_1_2_10_66_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(56)90204-1"},{"key":"e_1_2_10_67_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.201900599"},{"key":"e_1_2_10_68_1","doi-asserted-by":"publisher","DOI":"10.1002\/cctc.201000094"},{"key":"e_1_2_10_69_1","doi-asserted-by":"publisher","DOI":"10.3109\/07388551.2014.900734"},{"key":"e_1_2_10_70_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.60383"},{"key":"e_1_2_10_71_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00526-06"},{"key":"e_1_2_10_72_1","doi-asserted-by":"publisher","DOI":"10.3389\/fctls.2021.780474"},{"key":"e_1_2_10_73_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-1097.1998.tb02488.x"},{"key":"e_1_2_10_74_1","doi-asserted-by":"publisher","DOI":"10.1021\/acscatal.0c03755"},{"key":"e_1_2_10_75_1","doi-asserted-by":"publisher","DOI":"10.1042\/BSR20181210"},{"key":"e_1_2_10_76_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01494406"},{"key":"e_1_2_10_77_1","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-148-6-1607"},{"key":"e_1_2_10_78_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.70.6.3566-3574.2004"},{"key":"e_1_2_10_79_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2017.12.003"},{"key":"e_1_2_10_80_1","first-page":"11","article-title":"Zur quantitative Analyse der Chloroplastenpigmente. I. Kritische \u00dcberpr\u00fcfung der spektralphotometrischen Chlorophyll\u2010Bestimmung","volume":"41","author":"Ziegler R.","year":"1965","journal-title":"Beitr\u00e4ge Zur Biologie der Pflanzen"}],"container-title":["Plant Direct"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pld3.480","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/pld3.480","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pld3.480","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,20]],"date-time":"2023-08-20T16:46:57Z","timestamp":1692550017000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/pld3.480"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,1]]},"references-count":79,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2023,1]]}},"alternative-id":["10.1002\/pld3.480"],"URL":"http:\/\/dx.doi.org\/10.1002\/pld3.480","archive":["Portico"],"relation":{},"ISSN":["2475-4455","2475-4455"],"issn-type":[{"value":"2475-4455","type":"print"},{"value":"2475-4455","type":"electronic"}],"subject":["Plant Science","Biochemistry, Genetics and Molecular Biology (miscellaneous)","Ecology","Ecology, Evolution, Behavior and Systematics"],"published":{"date-parts":[[2023,1]]},"assertion":[{"value":"2022-06-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-12-31","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-01-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}} |