1 line
19 KiB
Plaintext
1 line
19 KiB
Plaintext
{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,9,12]],"date-time":"2023-09-12T21:37:02Z","timestamp":1694554622705},"reference-count":42,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2023,4,19]],"date-time":"2023-04-19T00:00:00Z","timestamp":1681862400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100008789","name":"Department of Science and Technology, Government of West Bengal","doi-asserted-by":"publisher","award":["969\/(Sanc)\/ST\/P\/SandT\/9G-22\/2016"]},{"DOI":"10.13039\/501100001411","name":"Indian Council of Medical Research","doi-asserted-by":"publisher","award":["6\/9-7(151)2017-ECD II"]},{"name":"JC Bose Fellowship, Govt. of India","award":["JCB\/2019\/000043"]},{"name":"Department Of Science & Technology, Govt. of India - German Academic Exchange Service","award":["DST\/INT\/DAAD\/P-13\/2020"]},{"name":"Department Of Science & Technology, Govt. of India - German Academic Exchange Service","award":["57588068"]}],"content-domain":{"domain":["www.plosntds.org"],"crossmark-restriction":false},"short-container-title":["PLoS Negl Trop Dis"],"abstract":"<jats:sec id=\"sec001\">\n<jats:title>Background<\/jats:title>\n<jats:p>The potential reservoirs of visceral leishmaniasis (VL) in South Asia include asymptomatic and relapsed cases of VL, along with patients with post kala-azar dermal leishmaniasis (PKDL). Accordingly, accurate estimation of their parasite load is pivotal for ensuring disease elimination, presently targeted for 2023. Serological tests cannot accurately detect relapses and\/or monitor treatment effectiveness, and therefore, parasite antigen\/nucleic acid based detection assays remain the only viable option. An excellent option is the quantitative polymerase chain reaction (qPCR) but the high cost, technical expertise and time involved precludes its wider acceptability. Accordingly, the recombinase polymerase amplification (RPA) assay operated in a mobile suitcase laboratory has emerged not simply as a diagnostic tool for leishmaniasis but also to monitor the disease burden.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec002\">\n<jats:title>Methodology\/Principal findings<\/jats:title>\n<jats:p>Using total genomic DNA isolated from peripheral blood of confirmed VL cases (n = 40) and lesional biopsies of PKDL cases (n = 64), the kinetoplast-DNA based qPCR and RPA assay was performed and parasite load expressed as Cycle threshold (Ct) and Time threshold (Tt) respectively. Using qPCR as the gold standard, the diagnostic specificity and sensitivity of RPA in na\u00efve cases of VL and PKDL was reiterated. To assess the prognostic potential of the RPA, samples were analyzed immediately at the end of treatment or \u22656 months following completion of treatment. In cases of VL, the RPA assay in terms of cure and detection of a relapse case showed 100% concordance with qPCR. In PKDL following completion of treatment, the overall detection concordance between RPA and qPCR was 92.7% (38\/41). At the end of treatment for PKDL, 7 cases remained qPCR positive, whereas RPA was positive in only 4\/7 cases, perhaps attributable to their low parasite load.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec003\">\n<jats:title>Conclusions\/Significance<\/jats:title>\n<jats:p>This study endorsed the potential of RPA to evolve as a field applicable, molecular tool for monitoring parasite load, possibly at a point of care level and is worthy of consideration in resource limited settings.<\/jats:p>\n<\/jats:sec>","DOI":"10.1371\/journal.pntd.0011231","type":"journal-article","created":{"date-parts":[[2023,4,19]],"date-time":"2023-04-19T17:32:48Z","timestamp":1681925568000},"page":"e0011231","update-policy":"http:\/\/dx.doi.org\/10.1371\/journal.pntd.corrections_policy","source":"Crossref","is-referenced-by-count":1,"title":["Evaluation of Recombinase Polymerase Amplification assay for monitoring parasite load in patients with kala-azar and post kala-azar dermal leishmaniasis"],"prefix":"10.1371","volume":"17","author":[{"given":"Madhurima","family":"Roy","sequence":"first","affiliation":[]},{"given":"Arianna","family":"Ceruti","sequence":"additional","affiliation":[]},{"given":"Rea Maja","family":"Kobialka","sequence":"additional","affiliation":[]},{"given":"Sutopa","family":"Roy","sequence":"additional","affiliation":[]},{"given":"Deblina","family":"Sarkar","sequence":"additional","affiliation":[]},{"given":"Ahmed Abd El","family":"Wahed","sequence":"additional","affiliation":[]},{"ORCID":"http:\/\/orcid.org\/0000-0002-5123-6019","authenticated-orcid":true,"given":"Mitali","family":"Chatterjee","sequence":"additional","affiliation":[]}],"member":"340","published-online":{"date-parts":[[2023,4,19]]},"reference":[{"key":"pntd.0011231.ref001","doi-asserted-by":"crossref","first-page":"494","DOI":"10.1016\/S1473-3099(02)00347-X","article-title":"Visceral leishmaniasis: current status of control, diagnosis, and treatment, and a proposed research and development agenda","volume":"2","author":"PJ Guerin","year":"2002","journal-title":"Lancet Infect Dis"},{"key":"pntd.0011231.ref002","doi-asserted-by":"crossref","first-page":"873","DOI":"10.1038\/nrmicro1748","article-title":"Visceral leishmaniasis: what are the needs for diagnosis, treatment and control","volume":"5","author":"F Chappuis","year":"2007","journal-title":"Nat Rev Microbiol"},{"key":"pntd.0011231.ref003","doi-asserted-by":"crossref","first-page":"951","DOI":"10.1016\/S0140-6736(18)31204-2","article-title":"Leishmaniasis","volume":"392","author":"S Burza","year":"2018","journal-title":"Lancet"},{"key":"pntd.0011231.ref004","doi-asserted-by":"crossref","first-page":"404","DOI":"10.1093\/cid\/cix808","article-title":"Monitoring of parasite kinetics in Indian post-kala-azar dermal leishmaniasis","volume":"66","author":"S Moulik","year":"2018","journal-title":"Clin Infect Dis"},{"key":"pntd.0011231.ref005","doi-asserted-by":"crossref","first-page":"e0007249","DOI":"10.1371\/journal.pntd.0007249","article-title":"Active surveillance identified a neglected burden of macular cases of post kala-azar dermal leishmaniasis in West Bengal.","volume":"13","author":"R Sengupta","year":"2019","journal-title":"PLoS Negl Trop Dis."},{"key":"pntd.0011231.ref006","volume-title":"Ending the neglect to attain the sustainable development goals: a road map for neglected tropical diseases 2021\u20132030","year":"2020"},{"key":"pntd.0011231.ref007","doi-asserted-by":"crossref","first-page":"805","DOI":"10.1080\/14787210.2018.1532790","article-title":"Visceral leishmaniasis elimination targets in India, strategies for preventing resurgence.","volume":"16","author":"S Sundar","year":"2018","journal-title":"Expert Rev Anti Infect Ther"},{"key":"pntd.0011231.ref008","doi-asserted-by":"crossref","first-page":"S102","DOI":"10.1038\/nature16042","article-title":"Health-seeking behaviour, diagnostics and transmission dynamics in the control of visceral leishmaniasis in the Indian subcontinent","volume":"528","author":"GF Medley","year":"2015","journal-title":"Nature"},{"key":"pntd.0011231.ref009","doi-asserted-by":"crossref","first-page":"24","DOI":"10.4103\/ijd.IJD_602_20","article-title":"Post kala-azar dermal leishmaniasis: Clinical features and differential diagnosis","volume":"66","author":"P Kumar","year":"2021","journal-title":"Indian J Dermatol"},{"key":"pntd.0011231.ref010","doi-asserted-by":"crossref","first-page":"623437","DOI":"10.3389\/fcimb.2021.623437","article-title":"Molecular tracking of the Leishmania parasite","volume":"11","author":"S Moulik","year":"2021","journal-title":"Front Cell Infect Microbiol"},{"key":"pntd.0011231.ref011","doi-asserted-by":"crossref","first-page":"e204","DOI":"10.1371\/journal.pbio.0040204","article-title":"DNA detection using recombination proteins","volume":"4","author":"O Piepenburg","year":"2006","journal-title":"PLoS Biol"},{"key":"pntd.0011231.ref012","doi-asserted-by":"crossref","article-title":"Reverse transcription recombinase polymerase amplification assay for the detection of middle east respiratory syndrome coronavirus.","author":"A Abd El Wahed","year":"2013","journal-title":"PLoS Curr.","DOI":"10.1371\/currents.outbreaks.62df1c7c75ffc96cd59034531e2e8364"},{"key":"pntd.0011231.ref013","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.jcv.2015.05.004","article-title":"Diagnostics-in-a-suitcase: development of a portable and rapid assay for the detection of the emerging avian influenza A (H7N9) virus","volume":"69","author":"A Abd El Wahed","year":"2015","journal-title":"J Clin Virol."},{"key":"pntd.0011231.ref014","doi-asserted-by":"crossref","first-page":"e0129682","DOI":"10.1371\/journal.pone.0129682","article-title":"Recombinase polymerase amplification assay for rapid diagnostics of dengue infection.","volume":"10","author":"A Abd El Wahed","year":"2015","journal-title":"PLoS One"},{"key":"pntd.0011231.ref015","doi-asserted-by":"crossref","DOI":"10.2807\/1560-7917.ES.2015.20.44.30053","article-title":"Development and deployment of a rapid recombinase polymerase amplification ebola virus detection assay in Guinea in 2015.","volume":"20","author":"O Faye","year":"2015","journal-title":"Euro Surveill."},{"key":"pntd.0011231.ref016","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.diagmicrobio.2019.03.015","article-title":"Recombinase polymerase amplification assay for rapid detection of monkeypox virus","volume":"95","author":"SD Davi","year":"2019","journal-title":"Diagn Microbiol Infect Dis"},{"key":"pntd.0011231.ref017","doi-asserted-by":"crossref","first-page":"1237","DOI":"10.3390\/pathogens10101237","article-title":"Molecular detection of feline coronavirus based on recombinase polymerase amplification assay.","volume":"10","author":"RM Kobialka","year":"2021","journal-title":"Pathogens."},{"key":"pntd.0011231.ref018","doi-asserted-by":"crossref","first-page":"3131","DOI":"10.1038\/s41598-021-82479-8","article-title":"A recombinase polymerase amplification assay for rapid detection of rabies virus.","volume":"11","author":"M Faye","year":"2021","journal-title":"Sci Rep"},{"key":"pntd.0011231.ref019","doi-asserted-by":"crossref","first-page":"665","DOI":"10.1186\/s13071-018-3238-1","article-title":"Evaluation of rapid extraction and isothermal amplification techniques for the detection of Leishmania donovani DNA from skin lesions of suspected cases at the point of need","volume":"11","author":"G Gunaratna","year":"2018","journal-title":"Sri Lanka. Parasit Vectors"},{"key":"pntd.0011231.ref020","doi-asserted-by":"crossref","first-page":"95","DOI":"10.3390\/tropicalmed5020095","article-title":"Evaluation of rapid extraction methods coupled with a recombinase polymerase amplification assay for point-of-need diagnosis of post-kala-azar dermal leishmaniasis","volume":"5","author":"R Chowdhury","year":"2020","journal-title":"Trop Med Infect Dis"},{"key":"pntd.0011231.ref021","doi-asserted-by":"crossref","first-page":"1963","DOI":"10.3390\/diagnostics11111963","article-title":"Development of quantitative rapid isothermal amplification assay for Leishmania donovani","volume":"11","author":"MAA Khan","year":"2021","journal-title":"Diagnostics"},{"key":"pntd.0011231.ref022","doi-asserted-by":"crossref","first-page":"588","DOI":"10.3390\/microorganisms9030588","article-title":"A multi-country, single-blinded, phase 2 study to evaluate a point-of-need system for rapid detection of leishmaniasis and its implementation in endemic settings.","volume":"9","author":"P Ghosh","year":"2021","journal-title":"Microorganisms."},{"key":"pntd.0011231.ref023","doi-asserted-by":"crossref","first-page":"688","DOI":"10.4269\/ajtmh.2011.10-0590","article-title":"Case series of misdiagnosis with rK39 strip test in Indian leishmaniasis","volume":"84","author":"NK Das","year":"2011","journal-title":"Am J Trop Med Hyg"},{"key":"pntd.0011231.ref024","doi-asserted-by":"crossref","first-page":"921","DOI":"10.1111\/j.1365-4632.2010.04558.x","article-title":"Post-kala-azar dermal leishmaniasis-an overview","volume":"49","author":"S Ganguly","year":"2010","journal-title":"Int J Dermatol"},{"key":"pntd.0011231.ref025","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1186\/s13071-016-1572-8","article-title":"Mobile suitcase laboratory for rapid detection of Leishmania donovani using recombinase polymerase amplification assay.","volume":"9","author":"D Mondal","year":"2016","journal-title":"Parasit Vectors"},{"key":"pntd.0011231.ref026","doi-asserted-by":"crossref","first-page":"e1763","DOI":"10.1016\/S2214-109X(21)00392-2","article-title":"Towards the elimination of visceral leishmaniasis as a public health problem in east Africa: reflections on an enhanced control strategy and a call for action","volume":"9","author":"J Alvar","year":"2021","journal-title":"Lancet Glob Health"},{"key":"pntd.0011231.ref027","doi-asserted-by":"crossref","first-page":"134","DOI":"10.3390\/vetsci8070134","article-title":"Reverse transcription recombinase polymerase amplification assay for rapid detection of avian influenza virus H9N2 HA gene","volume":"8","author":"N Yehia","year":"2021","journal-title":"Vet Sci"},{"key":"pntd.0011231.ref028","doi-asserted-by":"crossref","first-page":"e0004953","DOI":"10.1371\/journal.pntd.0004953","article-title":"A field-deployable reverse transcription recombinase polymerase amplification assay for rapid detection of the chikungunya virus.","volume":"10","author":"P Patel","year":"2016","journal-title":"PLoS Negl Trop Dis"},{"key":"pntd.0011231.ref029","doi-asserted-by":"crossref","first-page":"40244","DOI":"10.1038\/srep40244","article-title":"Development of a recombinase polymerase amplification assay for detection of epidemic human noroviruses","volume":"7","author":"MD Moore","year":"2017","journal-title":"Sci Rep"},{"key":"pntd.0011231.ref030","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1186\/s12879-015-1260-x","article-title":"Post-kala-azar dermal leishmaniasis and leprosy: case report and literature review.","volume":"15","author":"MA Trindade","year":"2015","journal-title":"BMC Infect Dis"},{"key":"pntd.0011231.ref031","doi-asserted-by":"crossref","first-page":"12491","DOI":"10.1021\/acs.chemrev.5b00428","article-title":"Isothermal amplification of nucleic acids","volume":"115","author":"Y Zhao","year":"2015","journal-title":"Chem Rev"},{"key":"pntd.0011231.ref032","doi-asserted-by":"crossref","first-page":"e0007698","DOI":"10.1371\/journal.pntd.0007698","article-title":"Loop-mediated isothermal amplification (LAMP): an advanced molecular point-of-care technique for the detection of Leishmania infection.","volume":"13","author":"CO Nzelu","year":"2019","journal-title":"PLoS Negl Trop Dis"},{"key":"pntd.0011231.ref033","doi-asserted-by":"crossref","first-page":"5560","DOI":"10.1128\/JCM.43.11.5560-5566.2005","article-title":"Quantitative nucleic acid sequence-based assay as a new molecular tool for detection and quantification of Leishmania parasites in skin biopsy samples","volume":"43","author":"WF van der Meide","year":"2005","journal-title":"J Clin Microbiol"},{"key":"pntd.0011231.ref034","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.actatropica.2013.12.016","article-title":"Development of a loop-mediated isothermal amplification method for rapid mass-screening of sand flies for Leishmania infection","volume":"132","author":"CO Nzelu","year":"2014","journal-title":"Acta Trop"},{"key":"pntd.0011231.ref035","doi-asserted-by":"crossref","first-page":"1325","DOI":"10.4269\/ajtmh.17-0808","article-title":"Analytical performance of a loop-mediated isothermal amplification assay for Leishmania DNA detection in sandflies and direct smears of patients with cutaneous leishmaniasis","volume":"98","author":"CM Le\u00f3n","year":"2018","journal-title":"Am J Trop Med Hyg"},{"key":"pntd.0011231.ref036","doi-asserted-by":"crossref","first-page":"2097","DOI":"10.4269\/ajtmh.20-1057","article-title":"Real-time fluorimetry loop-mediated isothermal amplification for diagnosis of leishmaniasis and as a tool for assessment of cure for post-kala-azar dermal leishmaniasis","volume":"104","author":"KK Dixit","year":"2021","journal-title":"Am J Trop Med Hyg"},{"key":"pntd.0011231.ref037","doi-asserted-by":"crossref","first-page":"e0013221","DOI":"10.1128\/JCM.00132-21","article-title":"Utility of blood as the clinical specimen for the molecular diagnosis of post-kala-azar dermal leishmaniasis","volume":"59","author":"KK Dixit","year":"2021","journal-title":"J Clin Microbiol"},{"key":"pntd.0011231.ref038","doi-asserted-by":"crossref","first-page":"15972","DOI":"10.1038\/s41598-018-34275-0","article-title":"Genome-informed diagnostics for specific and rapid detection of pectobacterium species using recombinase polymerase amplification coupled with a lateral flow device.","volume":"8","author":"FA Ahmed","year":"2018","journal-title":"Sci Rep"},{"key":"pntd.0011231.ref039","doi-asserted-by":"crossref","first-page":"e3273","DOI":"10.1371\/journal.pntd.0003273","article-title":"IgG1 as a potential biomarker of post-chemotherapeutic relapse in visceral leishmaniasis, and adaptation to a rapid diagnostic test.","volume":"8","author":"T Bhattacharyya","year":"2014","journal-title":"PLoS Negl Trop Dis"},{"key":"pntd.0011231.ref040","doi-asserted-by":"crossref","first-page":"e0009906","DOI":"10.1371\/journal.pntd.0009906","article-title":"IgG3 and IL10 are effective biomarkers for monitoring therapeutic effectiveness in post kala-azar dermal leishmaniasis.","volume":"15","author":"S Sengupta","year":"2021","journal-title":"PLoS Negl Trop Dis."},{"key":"pntd.0011231.ref041","doi-asserted-by":"crossref","first-page":"2268","DOI":"10.3389\/fmicb.2019.02268","article-title":"Relationship of serum antileishmanial antibody with development of visceral leishmaniasis, post-kala-azar dermal leishmaniasis and visceral leishmaniasis relapse.","volume":"10","author":"D Mondal","year":"2019","journal-title":"Front Microbiol."},{"key":"pntd.0011231.ref042","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1517\/14656566.2013.755515","article-title":"Leishmaniasis: an update of current pharmacotherapy.","volume":"14","author":"S Sundar","year":"2013","journal-title":"Expert Opin Pharmacother"}],"container-title":["PLOS Neglected Tropical Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pntd.0011231","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,4,19]],"date-time":"2023-04-19T17:33:18Z","timestamp":1681925598000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pntd.0011231"}},"subtitle":[],"editor":[{"given":"Michael W.","family":"Gaunt","sequence":"first","affiliation":[]}],"short-title":[],"issued":{"date-parts":[[2023,4,19]]},"references-count":42,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2023,4,19]]}},"URL":"http:\/\/dx.doi.org\/10.1371\/journal.pntd.0011231","relation":{},"ISSN":["1935-2735"],"issn-type":[{"value":"1935-2735","type":"electronic"}],"subject":["Infectious Diseases","Public Health, Environmental and Occupational Health"],"published":{"date-parts":[[2023,4,19]]}}} |