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{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,4,8]],"date-time":"2023-04-08T05:07:51Z","timestamp":1680930471074},"reference-count":40,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2023,4,7]],"date-time":"2023-04-07T00:00:00Z","timestamp":1680825600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Institute of Basic Medical Sciences, University of Oslo"},{"name":"Throne Holst Foundation"},{"name":"Western Norway Regional Health Authority"},{"name":"Deutsche Forschungsgemeinschaft","award":["209933838\u2014SFB 1052"]},{"name":"Deutsches Zentrum f\u00fcr Diabetesforschung","award":["82DZD00601"]},{"name":"Trond Mohn Stiftelse"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Proteoglycans are central components of the extracellular matrix (ECM) and binding partners for inflammatory chemokines. Morphological differences in the ECM and increased inflammation are prominent features of the white adipose tissues in patients with obesity. The impact of obesity and weight loss on the expression of specific proteoglycans in adipose tissue is not well known. This study aimed to investigate the relationship between adiposity and proteoglycan expression. We analyzed transcriptomic data from two human bariatric surgery cohorts. In addition, RT-qPCR was performed on adipose tissues from female and male mice fed a high-fat diet. Both visceral and subcutaneous adipose tissue depots were analyzed. Adipose mRNA expression of specific proteoglycans, proteoglycan biosynthetic enzymes, proteoglycan partner molecules, and other ECM-related proteins were altered in both human cohorts. We consistently observed more profound alterations in gene expression of ECM targets in the visceral adipose tissues after surgery (among others VCAN (p = 0.000309), OGN (p = 0.000976), GPC4 (p = 0.00525), COL1A1 (p = 0.00221)). Further, gene analyses in mice revealed sex differences in these two tissue compartments in obese mice. We suggest that adipose tissue repair is still in progress long after surgery, which may reflect challenges in remodeling increased adipose tissues. This study can provide the basis for more mechanistic studies on the role of proteoglycans in adipose tissues in obesity.<\/jats:p>","DOI":"10.3390\/ijms24086884","type":"journal-article","created":{"date-parts":[[2023,4,7]],"date-time":"2023-04-07T08:08:03Z","timestamp":1680854883000},"page":"6884","source":"Crossref","is-referenced-by-count":0,"title":["Obesity Is Associated with Distorted Proteoglycan Expression in Adipose Tissue"],"prefix":"10.3390","volume":"24","author":[{"given":"Astri J.","family":"Meen","sequence":"first","affiliation":[{"name":"Department of Medical Biology, UIT The Arctic University of Norway, 9019 Troms\u00f8, Norway"}]},{"given":"Atanaska I.","family":"Doncheva","sequence":"additional","affiliation":[{"name":"Department of Nutrition, University of Oslo, 0316 Oslo, Norway"}]},{"given":"Yvonne","family":"B\u00f6ttcher","sequence":"additional","affiliation":[{"name":"EpiGen, Medical Division, Akershus University Hospital, 1474 Nordbyhagen, Norway"},{"name":"Department of Endocrinology, Division of Medicine, Akershus University Hospital, 1478 L\u00f8renskog, Norway"}]},{"ORCID":"http:\/\/orcid.org\/0000-0001-6972-1824","authenticated-orcid":false,"given":"Simon N.","family":"Dankel","sequence":"additional","affiliation":[{"name":"Mohn Nutrition Research Laboratory, Department of Clinical Science, University of Bergen, 5020 Bergen, Norway"},{"name":"Hormone Laboratory, Department of Medical Biochemistry and Pharmacology, Haukeland University Hospital, 5020 Bergen, Norway"}]},{"ORCID":"http:\/\/orcid.org\/0000-0003-3258-3397","authenticated-orcid":false,"given":"Anne","family":"Hoffmann","sequence":"additional","affiliation":[{"name":"Helmholtz Institute for Metabolic, Obesity and Vascular Research (HI-MAG) of the Helmholtz Zentrum M\u00fcnchen at the University of Leipzig and University Hospital Leipzig, 04103 Leipzig, Germany"}]},{"ORCID":"http:\/\/orcid.org\/0000-0003-0208-2065","authenticated-orcid":false,"given":"Matthias","family":"Bl\u00fcher","sequence":"additional","affiliation":[{"name":"Helmholtz Institute for Metabolic, Obesity and Vascular Research (HI-MAG) of the Helmholtz Zentrum M\u00fcnchen at the University of Leipzig and University Hospital Leipzig, 04103 Leipzig, Germany"},{"name":"Medical Department III\u2014Endocrinology, Nephrology, Rheumatology, University of Leipzig Medical Center, 04103 Leipzig, Germany"}]},{"given":"Johan","family":"Fern\u00f8","sequence":"additional","affiliation":[{"name":"Mohn Nutrition Research Laboratory, Department of Clinical Science, University of Bergen, 5020 Bergen, Norway"},{"name":"Hormone Laboratory, Department of Medical Biochemistry and Pharmacology, Haukeland University Hospital, 5020 Bergen, Norway"}]},{"given":"Gunnar","family":"Mellgren","sequence":"additional","affiliation":[{"name":"Mohn Nutrition Research Laboratory, Department of Clinical Science, University of Bergen, 5020 Bergen, Norway"},{"name":"Hormone Laboratory, Department of Medical Biochemistry and Pharmacology, Haukeland University Hospital, 5020 Bergen, Norway"}]},{"ORCID":"http:\/\/orcid.org\/0000-0002-5160-4571","authenticated-orcid":false,"given":"Adhideb","family":"Ghosh","sequence":"additional","affiliation":[{"name":"Medical Department III\u2014Endocrinology, Nephrology, Rheumatology, University of Leipzig Medical Center, 04103 Leipzig, Germany"}]},{"given":"Wenfei","family":"Sun","sequence":"additional","affiliation":[{"name":"Institute of Food, Nutrition and Health, ETH Zurich, 8092 Schwerzenbach, Switzerland"}]},{"given":"Hua","family":"Dong","sequence":"additional","affiliation":[{"name":"Institute of Food, Nutrition and Health, ETH Zurich, 8092 Schwerzenbach, Switzerland"}]},{"ORCID":"http:\/\/orcid.org\/0009-0002-3360-5455","authenticated-orcid":false,"given":"Falko","family":"No\u00e9","sequence":"additional","affiliation":[{"name":"Institute of Food, Nutrition and Health, ETH Zurich, 8092 Schwerzenbach, Switzerland"}]},{"given":"Christian","family":"Wolfrum","sequence":"additional","affiliation":[{"name":"Institute of Food, Nutrition and Health, ETH Zurich, 8092 Schwerzenbach, Switzerland"}]},{"given":"Gunnar","family":"Pejler","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry and Microbiology, Uppsala University, 75123 Uppsala, Sweden"}]},{"given":"Knut Tomas","family":"Dalen","sequence":"additional","affiliation":[{"name":"Department of Nutrition, University of Oslo, 0316 Oslo, Norway"}]},{"given":"Svein O.","family":"Kolset","sequence":"additional","affiliation":[{"name":"Department of Nutrition, University of Oslo, 0316 Oslo, Norway"}]}],"member":"1968","published-online":{"date-parts":[[2023,4,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"721","DOI":"10.1203\/01.PDR.0000059753.61905.58","article-title":"Genetics and the Pathophysiology of Obesity","volume":"53","year":"2003","journal-title":"Pediatr. 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