uni-leipzig-open-access/json/cyto.a.24766

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The panel covers all main leukocyte populations: neutrophils, eosinophils and basophils, monocytes (with additional subsets), dendritic cells, innate lymphoid cells and lymphocytes. As for lymphocytes, this panel includes CD4+ T helper, Treg cells, and CD8+ cytotoxic T cells. Further T cells subsets are included with special focus on invariant T cells: \u03b3\u03b4 T cells (including \u03b42TCR variant), invariant NKT cells and MAIT (mucosal\u2010associated invariant T cells) cells. Additionally, total B cells (including Bregs and plasmocytes), NK cells, and NKT cells are included. For the overall check of activation status of the analyzed immune cells we used HLA\u2010DR, CD38, CD57, CD69, PD\u20101, and CD94. In addition, we used CD62L, CD45RA, CD27, and CD39 to describe the differentiation status of these cells. The panel was designed to maximize the information that can be obtained from surface markers in order to avoid the need for fixation and permeabilization steps. The presented multimarker panel offers the possibility to discover new immune cell subtypes which in patients and in cohort studies may lead to the identification of altered immune phenotypes and might give a link to immune system based or to certain other diseases. This panel was developed for a full spectrum flow cytometer equipped with a minimum of three lasers. We developed this panel using healthy human fresh blood, however it was also successfully used for staining of isolated human peripheral blood mononuclear cells (PBMC).<\/jats:p>","DOI":"10.1002\/cyto.a.24766","type":"journal-article","created":{"date-parts":[[2023,5,31]],"date-time":"2023-05-31T06:31:35Z","timestamp":1685514695000},"page":"695-702","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["OMIP\u201094: Twenty\u2010four\u2010color (thirty\u2010marker) panel for deep immunophenotyping of immune cells in human peripheral blood"],"prefix":"10.1002","volume":"103","author":[{"given":"Arkadiusz","family":"Pierzchalski","sequence":"first","affiliation":[{"name":"Department of Environmental Immunology Helmholtz Centre for Environmental Research\u2013UFZ  Leipzig Germany"}]},{"given":"Ana C.","family":"Zenclussen","sequence":"additional","affiliation":[{"name":"Department of Environmental Immunology Helmholtz 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