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{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,1,3]],"date-time":"2024-01-03T08:04:34Z","timestamp":1704269074603},"reference-count":91,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2022,11,29]],"date-time":"2022-11-29T00:00:00Z","timestamp":1669680000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001655","name":"Deutscher Akademischer Austauschdienst","doi-asserted-by":"publisher"},{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","award":["AGRIPOLY"]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["2020.00758.CEECIND"]},{"DOI":"10.13039\/501100006111","name":"Minist\u00e9rio da Ci\u00eancia, Tecnologia e Ensino Superior","doi-asserted-by":"publisher","award":["PTDC\/QUI\u2010OUT\/30658\/2017","CENTRO\u201001\u20100145\u2010FEDER\u2010030658"]},{"DOI":"10.13039\/501100003381","name":"Minist\u00e9rio da Educa\u00e7\u00e3o e Ci\u00eancia","doi-asserted-by":"publisher"},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["396823779"]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Adv Healthcare Materials"],"published-print":{"date-parts":[[2023,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A gene\u2010activated surface coating is presented as a strategy to design smart biomaterials for bone tissue engineering. The thin\u2010film coating is based on polyelectrolyte multilayers\u00a0composed of collagen I and chondroitin sulfate, two main biopolymers of the bone extracellular matrix, which are fabricated by layer\u2010by\u2010layer assembly. For further functionalization, DNA\/lipid\u2010nanoparticles (lipoplexes) are incorporated into the multilayers. The polyelectrolyte multilayer fabrication and lipoplex deposition are analyzed by surface sensitive analytical methods that demonstrate successful thin\u2010film formation, fibrillar structuring of collagen, and homogenous embedding of lipoplexes. Culture of mesenchymal stem cells on the lipoplex functionalized multilayer results in excellent attachment and growth of them, and also, their ability to take up cargo like fluorescence\u2010labelled DNA from lipoplexes. The functionalization of the multilayer with lipoplexes encapsulating DNA encoding for transient expression of bone morphogenetic protein 2 induces osteogenic differentiation of mesenchymal stem cells, which is shown by mRNA quantification for osteogenic genes and histochemical staining. In summary, the novel gene\u2010functionalized and extracellular matrix mimicking multilayer composed of collagen I, chondroitin sulfate, and lipoplexes, represents a smart surface functionalization that holds great promise for tissue engineering constructs and implant coatings to promote regeneration of bone and other tissues.<\/jats:p>","DOI":"10.1002\/adhm.202201978","type":"journal-article","created":{"date-parts":[[2022,11,15]],"date-time":"2022-11-15T08:48:40Z","timestamp":1668502120000},"update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Lipoplex\u2010Functionalized Thin\u2010Film Surface Coating Based on Extracellular Matrix Components as Local Gene Delivery System to Control Osteogenic Stem Cell Differentiation"],"prefix":"10.1002","volume":"12","author":[{"given":"Catharina","family":"Husteden","sequence":"first","affiliation":[{"name":"Institute of Pharmacy Department of Medicinal Chemistry Martin Luther University Halle\u2010Wittenberg Wolfgang\u2010Langenbeck\u2010Str. 4 06120 Halle (Saale) Germany"}]},{"given":"Yazmin A.","family":"Brito Barrera","sequence":"additional","affiliation":[{"name":"Institute of Pharmacy Department of Biomedical Materials Martin Luther University Halle\u2010Wittenberg Hei
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