{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,7,4]],"date-time":"2023-07-04T04:39:42Z","timestamp":1688445582834},"reference-count":49,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2023,7,1]],"date-time":"2023-07-01T00:00:00Z","timestamp":1688169600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Sciences"],"abstract":"<jats:p>Miniature Schnauzers are predisposed to develop pancreatitis, with familial hypertriglyceridemia (HTG) described as a potential risk factor. Diagnosing pancreatitis in dogs is based on the integration of serum canine-specific pancreatic lipase (cPLI) concentration, clinical presentation, and diagnostic imaging findings. However, markers of systemic inflammation and antiprotease activity have not been extensively investigated in the characterization and prognostication of pancreatitis in dogs. Serum concentrations of alpha1-proteinase inhibitor (\u03b11PI; as a marker of systemic antiprotease response) and calprotectin and S100A12 (as markers of systemic inflammation) were measured in serum samples from 35 Miniature Schnauzers diagnosed with pancreatitis (serum cPLI concentration &gt;400 \u03bcg\/L, clinical signs, abdominal imaging findings). These markers were evaluated for possible associations with patient characteristics, clinical presentation, risk factors for pancreatitis, and outcome. The study showed that biomarkers of systemic inflammation and antiprotease activity are commonly increased in Miniature Schnauzers with pancreatitis. Whereas serum calprotectin and S100A12 concentrations were found to have limited utility in differentiating pancreatitis presentations, serum \u03b11PI concentrations and potentially also the serum calprotectin-to-S100A12 ratio might be non-invasive surrogate markers of disease severity in dogs with pancreatitis.<\/jats:p>","DOI":"10.3390\/vetsci10070428","type":"journal-article","created":{"date-parts":[[2023,7,3]],"date-time":"2023-07-03T04:49:27Z","timestamp":1688359767000},"page":"428","source":"Crossref","is-referenced-by-count":0,"title":["Serum \u03b11-Proteinase Inhibitor, Calprotectin, and S100A12 Concentrations in the Characterization of Pancreatitis in Dogs"],"prefix":"10.3390","volume":"10","author":[{"given":"Annina N.","family":"Jandel","sequence":"first","affiliation":[{"name":"Department for Small Animals, College of Veterinary Medicine, University of Leipzig, DE-04103 Leipzig, Germany"}]},{"ORCID":"http:\/\/orcid.org\/0000-0003-3485-5157","authenticated-orcid":false,"given":"Romy M.","family":"Heilmann","sequence":"additional","affiliation":[{"name":"Department for Small Animals, College of Veterinary Medicine, University of Leipzig, DE-04103 Leipzig, Germany"},{"name":"Gastrointestinal Laboratory, School of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX 77843, USA"}]},{"given":"Henri","family":"Sander","sequence":"additional","affiliation":[{"name":"Department for Small Animals, College of Veterinary Medicine, University of Leipzig, DE-04103 Leipzig, Germany"}]},{"given":"J\u00f6rg M.","family":"Steiner","sequence":"additional","affiliation":[{"name":"Gastrointestinal Laboratory, School of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX 77843, USA"}]},{"given":"Niels","family":"Gr\u00fctzner","sequence":"additional","affiliation":[{"name":"Ruminant and Swine Clinic, Free University of Berlin, DE-14165 Berlin, Germany"},{"name":"VetaRegio GmbH, DE-06366 K\u00f6then, Germany"}]},{"ORCID":"http:\/\/orcid.org\/0000-0002-9508-0271","authenticated-orcid":false,"given":"Panagiotis G.","family":"Xenoulis","sequence":"additional","affiliation":[{"name":"Gastrointestinal Laboratory, School of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX 77843, USA"},{"name":"Clinic of Medicine, Faculty of Veterinary Science, University of Thessaly, 43100 Karditsa, Greece"}]}],"member":"1968","published-online":{"date-parts":[[2023,7,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"875","DOI":"10.1111\/j.1939-1676.2012.00949.x","article-title":"Pathophysiology of acute pancreatitis: Potential application from experimental models and human medicine to dogs","volume":"26","author":"Mansfield","year":"2012","journal-title":"J. Vet. Intern. Med."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"636","DOI":"10.1111\/jvim.15693","article-title":"Association between abdominal ultrasound findings, the specific canine pancreatic lipase assay, clinical severity indices, and clinical diagnosis in dogs with pancreatitis","volume":"34","author":"Cridge","year":"2020","journal-title":"J. Vet. Intern. Med."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1111\/jsap.12274","article-title":"Diagnosis of pancreatitis in dogs and cats","volume":"56","author":"Xenoulis","year":"2015","journal-title":"J. Small Anim. Pract."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"312","DOI":"10.1111\/j.1939-165X.2012.00458.x","article-title":"Canine and feline pancreatic lipase immunoreactivity","volume":"41","author":"Xenoulis","year":"2012","journal-title":"Vet. Clin. Pathol."},{"key":"ref_5","first-page":"263","article-title":"Sensitivity of serum markers for pancreatitis in dogs with macroscopic evidence of pancreatitis","volume":"9","author":"Steiner","year":"2008","journal-title":"Vet. Ther."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1241","DOI":"10.1111\/j.1939-1676.2011.00793.x","article-title":"Sensitivity and specificity of canine pancreas-specific lipase (cPL) and other markers for pancreatitis in 70 dogs with and without histopathologic evidence of pancreatitis","volume":"25","author":"Trivedi","year":"2011","journal-title":"J. Vet. Intern. Med."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"2572","DOI":"10.1111\/jvim.16292","article-title":"Advances in the diagnosis of acute pancreatitis in dogs","volume":"35","author":"Cridge","year":"2021","journal-title":"J. Vet. Intern. Med."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1111\/jvim.12470","article-title":"Quantitative contrast-enhanced ultrasonographic assessment of naturally occurring pancreatitis in dogs","volume":"29","author":"Lim","year":"2015","journal-title":"J. Vet. Intern. Med."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1111\/jvim.15364","article-title":"Computed tomographic angiography and ultrasonography in the diagnosis and evaluation of acute pancreatitis in dogs","volume":"33","author":"French","year":"2019","journal-title":"J. Vet. Intern. Med."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/j.tvjl.2016.02.010","article-title":"New advances in the diagnosis of canine and feline liver and pancreatic disease","volume":"215","author":"Lidbury","year":"2016","journal-title":"Vet. J."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"282","DOI":"10.1038\/nrrheum.2011.37","article-title":"C-reactive protein in rheumatology: Biology and genetics","volume":"7","author":"Rhodes","year":"2011","journal-title":"Nat. Rev. Rheumatol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"936","DOI":"10.2460\/javma.233.6.936","article-title":"Development of a clinical severity index for dogs with acute pancreatitis","volume":"233","author":"Mansfield","year":"2008","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1111\/jsap.12297","article-title":"Serum paraoxonase 1 (PON1) activity in acute pancreatitis of dogs","volume":"56","author":"Tvarijonaviciute","year":"2015","journal-title":"J. Small Anim. Pract."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1080\/01652176.2019.1655178","article-title":"Evaluation of serum C-reactive protein and high mobility group box 1 concentrations in 22 dogs with acute pancreatitis: A pilot study","volume":"39","author":"Kim","year":"2019","journal-title":"Vet. Q."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"2187","DOI":"10.1111\/jvim.16218","article-title":"Serum concentrations of canine pancreatic lipase immunoreactivity and C-reactive protein for monitoring disease progression in dogs with acute pancreatitis","volume":"35","author":"Keany","year":"2021","journal-title":"J. Vet. Intern. Med."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1292\/jvms.16-0009","article-title":"Assessment of severity and changes in C-reactive protein concentration and various biomarkers in dogs with pancreatitis","volume":"79","author":"Sato","year":"2017","journal-title":"J. Vet. Med. Sci."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1080\/00480169.2020.1780995","article-title":"Evaluation of C-reactive protein\/albumin ratio and its relationship with survival in dogs with acute pancreatitis","volume":"68","author":"Gori","year":"2020","journal-title":"N. Z. Vet. J."},{"key":"ref_18","unstructured":"Ettinger, S.J., and Feldman, E.C. (2010). Textbook of Veterinary Internal Medicine, Elsevier Saunders. [7th ed.]."},{"key":"ref_19","first-page":"175","article-title":"Development and analytic validation of an enzyme-linked immunosorbent assay for the measurement of canine pancreatic lipase immunoreactivity in serum","volume":"67","author":"Steiner","year":"2003","journal-title":"Can. J. Vet. Res."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"229","DOI":"10.5326\/0460229","article-title":"Association between serum triglyceride and canine pancreatic lipase immunoreactivity concentrations in Miniature Schnauzers","volume":"46","author":"Xenoulis","year":"2010","journal-title":"J. Am. Anim. Hosp. Assoc."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1111\/j.1939-1676.2010.0644.x","article-title":"Serum triglyceride concentrations in Miniature Schnauzers with and without a history of probable pancreatitis","volume":"25","author":"Xenoulis","year":"2011","journal-title":"J. Vet. Intern. Med."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1224","DOI":"10.1111\/j.1939-1676.2007.tb01942.x","article-title":"Investigation of hypertriglyceridemia in healthy Miniature Schnauzers","volume":"21","author":"Xenoulis","year":"2007","journal-title":"J. Vet. Intern. Med."},{"key":"ref_23","first-page":"315","article-title":"Evaluation of the cationic trypsinogen gene for potential mutations in Miniature Schnauzers with pancreatitis","volume":"68","author":"Bishop","year":"2004","journal-title":"Can. J. Vet. Res."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"527","DOI":"10.2460\/ajvr.71.5.527","article-title":"Identifications of variants of the SPINK1 gene and their association with pancreatitis in Miniature Schnauzers","volume":"71","author":"Bishop","year":"2010","journal-title":"Am. J. Vet. Res."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1111\/jsap.12396","article-title":"Canine hyperlipidemia","volume":"56","author":"Xenoulis","year":"2015","journal-title":"J. Small Anim. Pract."},{"key":"ref_26","first-page":"46","article-title":"Evaluation of risk factors for fatal acute pancreatitis in dogs","volume":"214","author":"Hess","year":"1999","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"ref_27","first-page":"673","article-title":"Risk factors associated with acute pancreatitis in dogs: 101 cases (1985\u20131990)","volume":"203","author":"Cook","year":"1993","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1097\/00004836-200301000-00016","article-title":"Issues in hyperlipidemic pancreatitis","volume":"36","author":"Yadav","year":"2003","journal-title":"J. Clin. Gastroenterol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.tvjl.2008.10.011","article-title":"Lipid metabolism and hyperlipidemia in dogs","volume":"183","author":"Xenoulis","year":"2010","journal-title":"Vet. J."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/j.tvjl.2016.04.009","article-title":"Proteinuria and lipoprotein lipase activity in Miniature Schnauzer dogs with and without hypertriglyceridemia","volume":"212","author":"Furrow","year":"2016","journal-title":"Vet. J."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1011","DOI":"10.2460\/javma.238.8.1011","article-title":"Association of hypertriglyceridemia with insulin resistance in healthy Miniature Schnauzers","volume":"238","author":"Xenoulis","year":"2011","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"910","DOI":"10.1111\/jvim.16418","article-title":"Associations among serum insulin, calprotectin, and C-reactive protein concentrations in Miniature Schnauzers with idiopathic hyperlipidemia before and after feeding an ultra-low-fat diet","volume":"36","author":"Xenoulis","year":"2022","journal-title":"J. Vet. Intern. Med."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"578","DOI":"10.1111\/jvim.15460","article-title":"Association of serum calprotectin (S100A8\/A9) concentrations and idiopathic hyperlipidemia in Miniature Schnauzers","volume":"33","author":"Heilmann","year":"2019","journal-title":"J. Vet. Intern. Med."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"2605","DOI":"10.1111\/jvim.15880","article-title":"Effect of a low-fat diet on serum triglyceride and cholesterol concentrations and lipoprotein profiles in Miniature Schnauzers with hypertriglyceridemia","volume":"34","author":"Xenoulis","year":"2020","journal-title":"J. Vet. Intern. Med."},{"key":"ref_35","first-page":"376","article-title":"Serum concentrations of canine alpha1-proteinase inhibitor in Yorkshire Terriers with and without cobalamin deficiency","volume":"26","author":"Heilmann","year":"2012","journal-title":"J. Vet. Diagn. Investig."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/j.tvjl.2019.03.002","article-title":"Biological variation of serum canine calprotectin concentrations as measured by ELISA in healthy dogs","volume":"247","author":"Heilmann","year":"2019","journal-title":"Vet. J."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1016\/j.vetimm.2011.09.011","article-title":"Development and analytic validation of an immunoassay for the quantification of canine S100A12 in serum and fecal samples and its biological variability in serum from healthy dogs","volume":"144","author":"Heilmann","year":"2011","journal-title":"Vet. Immunol. Immunopathol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1111\/vcp.12039","article-title":"Serum alpha1-proteinase inhibitor concentrations in healthy dogs\u2014Method validation and determination of reference interval and intra-individual variation","volume":"42","author":"Heilmann","year":"2013","journal-title":"Vet. Clin. Pathol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1016\/S1096-2867(03)00072-0","article-title":"Diagnostic approaches to acute pancreatitis","volume":"18","author":"Ruaux","year":"2003","journal-title":"Clin. Tech. Small Anim. Pract."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1136\/bmj.313.7053.333","article-title":"Serum complex of trypsin 2 and alpha 1 antitrypsin as diagnostic and prognostic marker of acute pancreatitis: Clinical study in consecutive patients","volume":"313","author":"Hedstrom","year":"1996","journal-title":"BMJ"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"674","DOI":"10.1111\/vec.12674","article-title":"Serum alpha1-proteinase inhibitor concentrations in dogs with systemic inflammatory response syndrome or sepsis","volume":"27","author":"Heilmann","year":"2017","journal-title":"J. Vet. Emerg. Crit. Care"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1801","DOI":"10.1111\/jvim.15895","article-title":"Protease inhibitors, inflammatory markers, and their association with outcome in dogs with naturally occurring acute pancreatitis","volume":"34","author":"Kuzi","year":"2020","journal-title":"J. Vet. Intern. Med."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"779","DOI":"10.1080\/00365521.1984.12005808","article-title":"Protease inhibitors in acute human pancreatitis correlation between biochemical changes and clinical course","volume":"19","author":"Lasson","year":"1984","journal-title":"Scand. J. Gastroenterol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"195","DOI":"10.5505\/tjtes.2012.99075","article-title":"Alpha-1 protease inhibitor and antichymotrypsin levels in acute pancreatitis","volume":"18","author":"Kemik","year":"2012","journal-title":"Turk. J. Trauma Emerg. Surg."},{"key":"ref_45","first-page":"1119","article-title":"Trypsin-alpha1-protease inhibitor complexes in serum and clinical course of acute pancreatitis","volume":"19","author":"Lasson","year":"1984","journal-title":"Scand. J. Gastroenterol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1016\/j.rmed.2007.11.011","article-title":"Different expression ratio of S100A8\/A9 and S100A12 in acute and chronic lung diseases","volume":"102","author":"Lorenz","year":"2008","journal-title":"Respir. Med."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1016\/j.tvjl.2018.04.017","article-title":"Serum alpha1-proteinase inhibitor concentrations in dogs with exocrine pancreatic disease, chronic hepatitis or proteinuric chronic kidney disease","volume":"236","author":"Heilmann","year":"2018","journal-title":"Vet. J."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"804","DOI":"10.1111\/j.1751-0813.1998.tb12331.x","article-title":"A severity score for spontaneous canine acute pancreatitis","volume":"76","author":"Ruaux","year":"1998","journal-title":"Aust. Vet. J."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1111\/jvim.15421","article-title":"Development and validation of a novel clinical scoring system for short-term prediction of death in dogs with acute pancreatitis","volume":"33","author":"Dossin","year":"2019","journal-title":"J. Vet. Intern. Med."}],"container-title":["Veterinary Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2306-7381\/10\/7\/428\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,3]],"date-time":"2023-07-03T06:33:48Z","timestamp":1688366028000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2306-7381\/10\/7\/428"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,7,1]]},"references-count":49,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2023,7]]}},"alternative-id":["vetsci10070428"],"URL":"http:\/\/dx.doi.org\/10.3390\/vetsci10070428","relation":{},"ISSN":["2306-7381"],"issn-type":[{"value":"2306-7381","type":"electronic"}],"subject":["General Veterinary"],"published":{"date-parts":[[2023,7,1]]}}}